Figure 5 from Synthesis and Properties of Poly[N-(2-Hydroxypropyl) Methacrylamide] Conjugates of Superoxide Dismutase | Semantic Scholar (2024)

In vivo tests were performed by subcutaneous injection into mice and clearly demonstrated decreased proteolytic degradation for the protein-polymer conjugate when compared with native protein, indicating effective protein protection through a conjugation strategy.

This work proposes a new conjugation pathway and purification process in route to developing chromatographic alternatives to efficiently separate HPMA–protein conjugates and, the use of hydrophobic interaction chromatography (HIC) to separate polymer–protein Conjugates exploiting the hydrophobicity change on the protein surface due to the conjugations.

Non-targeted and targeted polymer–drug derivatives based on HPMA have both cytostatic and immunostimulating activity and their impressive anti-tumor effects most likely result from the combination of strong direct cytotoxicity and a systemic anticancer resistance regularly induced during the treatment.

The polymer-protein conjugates were characterized by HPLC, FPLC, and DLS and were seen to retain most of their native enzymatic activity, found to increase the circulation half-life compared with lysozyme alone.

PEG is the most used polymer and also the gold standard for stealth polymers in the emerging field of polymer-based drug delivery and alternative polymers will be evaluated.

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All the polymer-enzyme conjugates showed increased body residence time after intravenous injection in rats with respect to the native enzyme, and PVP was found to affect the pharmaco*kinetic properties of the enzyme after subcutaneous, intramuscular and intraperitoneal administration.

The modified ChT with poly(HPMA) significantly decreased the immunogenicity of ChT conjugates depending on the molecular weight of the poly( HPMA) and the degree of enzyme substitution.

It is suggested that pyran copolymer captured loosely bound Cu2+ during the reaction of SOD with H202, thus resulting in suppression of both 0OH-mediated inactivation of S OD and suppression of activity of a,-PI.

Modification of BS-RNase with poly(HPMA) can prevent it from degrading or inactivating events occurring in blood vessels after intravenous application, significantly enhancing its potential for therapeutical application.

A newly added property of the conjugate is cell-lubricating activity, which facilitated cell passage through micropores and reduced hemolysis during cell passage in vitro.

An important role played by cathepsin B in the degradation of polymeric substrates investigated in this study under physiological conditions is suggested and revealed the relationship between the structure of oligopeptide sequences and their degradability.

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